Control of Gene Expression, DNA Replication and Repair Start by reading the cour

Control of Gene Expression, DNA Replication and Repair
Start by reading the course textbook chapters and lesson for this week.
Define all key terms and concepts used in your responses.
Address each question in short written essay format.
Please refrain from the use images in your responses. You are expected to verbalize your responses in your own words and use sources for support. A point deduction will be applied for the use of images.
Use the course textbook and scholarly/credible sources as reference material. Each question should be answered completely and demonstrate an understanding of the concepts.
Each question will require the use of the textbook and other scholarly sources as reference material. Include both the in-text citations and reference list in APA format.
Purdue Owl APA
A minimum of five sentences will be required to provide an adequate response to each of the assignment questions to be considered for full credit.
Textbook Readings:
Karp, Chapters 11, 12 and 13
Helpful Links:
NCBI Bookshelf
Genome Biology
MR and BER
Question 1.
You’ve isolated a new protein from a cultured cell line. You have the proteins amino acid sequenced and learn that there are two very similar proteins. Their primary sequences are identical from the N-terminal end through about two thirds of the length of the proteins. Past that and continuing to the C-terminal end, the sequence is completely different and terminates a bit early on one of the proteins. Explain the what has occurred.
Question 2.
Eukaryotic messenger RNA includes a poly A tail. When the poly(A) tail is less than approximately 30 nucleotides in length, explain why the messenger RNA no longer is protected from nuclease digestion.
Question 3.
You have a mouse cell line that is deficient in nucleotide excision repair. You insert into this mouse cell line a gene fragment of human DNA suspected to carry the normal correlate of the mouse gene. You do this using a transfection assay. Describe, in detail, two ways that you can select, specifically for the mouse cells have taken up the gene fragment successfully.
Question 4.
Explain the reasons why a mutation in one of the genes that codes for a protein needed for mismatch repair would lead to elevated mutation rates in other genes.
***** This Source needs to be used for every question ******
Karp, G., Iwasa, J., & Marshall, W. (2015). Karp’s Cell and Molecular Biology. Concepts and Experiments.

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